Gene Expression Analysis
| The Jackson Laboratory Gene Expression Service was established with institutional funds, as well as support from the National Cancer Institute and a grant from The National Institute of Diabetes and Digestive and Kidney Diseases to enable Jackson staff to carry out large-scale gene expression analyses. Additional funding from the National Heart, Lung, and Blood Institute (NHLBI) ensures that NHLBI funded researchers and their collaborators have access to state of the art gene expression technology. |
The service offers RNA isolation, cDNA synthesis, fluorescently labeled cDNA for microarray analysis, and quantitative real-time PCR using the ABI 7700 and/or ABI 7900 platforms (including primers and probes design, experiment design and setup, and data analysis).
For microarray experiments, the Service recommends the Affymetrix GeneChip® platform. Using the 430 v2.0 Mouse Genome GeneChip, interrogation of over 39,000 transcripts is possible using this platform. Also offered is the Compugen Mouse Release 2.0 OligoLibrary™ (PDF) spotted microarray platform, which provides for the analysis of over 22,000 unique mouse genes.
A microarray database (MADJAX) to store microarray process information and experimental data has been implemented and is designed to adhere to Microarray Gene Expression Database Group standards. The service also carries out statistical analysis of array data. ANOVA programs for microarray analysis developed by Dr. Churchill's group are available.
The Service has Axon GenePix® 4000B and PerkinElmer ScanArray® Express HT microarray scanners, a high through-put Virtek Chipwriter™ Pro array printer, two Agilent 2100 Bioanalyzer instruments, a NanoDrop® ND-1000 for nucleic acid quantitation, a MJ Tetrad® thermocycler, a Qiagen Biorobot® 3000, and an Affymetrix GeneChip® workstation for instrumentation.
